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SOLVED:AMPPNP and the Contraction Cycle. AMPPNP is the abbreviation for a structural analogue of ATP in which the third phosphate group is linked to the second by a CH2 group instead of an oxygen atom. AMPPNP binds to the ATP-binding site of virtually all ATPases, including myosin. It differs from ATP, however, in that its terminal phosphate cannot be removed by hydrolysis. When isolated myofibrils are placed in a flask containing a solution of calcium ions and AMPPNP, contraction is quickly arr
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