Fluorescence microscopy is indispensable for visualizing biological structures and dynamics, yet its efficiency is limited—over half of emitted photons fall outside the objective’s numerical aperture ...

Light-sheet fluorescence microscopy (LSFM) has allowed for faster three-dimensional imaging of biological samples with lower photo-bleaching and photo-toxicity than confocal microscopy 1. This is ...

Selective plane illumination microscopy (SPIM) is a type of light sheet fluorescence microscopy (LSFM), which is one such 3D imaging technique employing a thin sheet of light to illuminate cleared ...

Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of the most significant developments in optics. Due to the wave nature of light ...

Confocal microscopy is a specialized fluorescence imaging technique that scientists use to acquire images at greater resolution than conventional microscopy. 1 In addition to scanning the lateral x ...

A hybrid microscope allows scientists to simultaneously image the full 3D orientation and position of an ensemble of molecules, such as labeled proteins inside cells. The microscope combines polarized ...